Kadar Flavonoid Total dan Aktivitas Antioksidan Ekstrak Etanol Angiopteris evecta (G. Forst.) Hoffm. dengan Metode ABTS

Total Flavonoid Content and Antioxidant Activity of Ethanolic Extract of Angiopteris evecta (G. Forst.) Hoffm. Using the ABTS Method

Authors

  • Muzayinah Muzayinah Program Studi Farmasi, STIKes KHAS Kempek, Cirebon, Indonesia
  • Yusfia Urwatul Wutsqa Program Studi Farmasi, STIKes KHAS Kempek, Cirebon, Indonesia
  • Fawwaz Muhammad Fauzi Program Studi Tadris Kimia, UIN Siber Syekh Nurjati Cirebon, Indonesia https://orcid.org/0000-0002-5756-8815

DOI:

https://doi.org/10.64689/jopanur.v1i02.10

Keywords:

Angiopteris evecta, kadar flavonoid total, aktivitas antioksidan, metode ABTS

Abstract

Angiopteris evecta (G. Forst.) Hoffm. merupakan tumbuhan paku dari famili Marattiaceae yang tersebar luas di wilayah tropis dan berpotensi sebagai sumber senyawa antioksidan alami. Penelitian ini bertujuan untuk mengidentifikasi kandungan metabolit sekunder, menentukan kadar flavonoid total, serta mengevaluasi aktivitas antioksidan ekstrak etanol A. evecta. Ekstraksi dilakukan menggunakan metode maserasi dengan pelarut etanol 96%. Skrining fitokimia dilakukan untuk mengidentifikasi golongan senyawa metabolit sekunder, meliputi alkaloid, flavonoid, fenolik, dan saponin. Penetapan kadar flavonoid total dilakukan menggunakan metode aluminium klorida (AlCl3) dengan kuersetin sebagai standar, yang dianalisis menggunakan spektrofotometer UV-Vis pada panjang gelombang maksimum. Aktivitas antioksidan diuji menggunakan metode ABTS (2,2’-azino-bis-(3-etilbenzotiazolin-6-asam sulfonat)) pada rentang konsentrasi 50–250 ppm, dengan kuersetin sebagai pembanding. Hasil skrining fitokimia menunjukkan bahwa ekstrak etanol A. evecta mengandung alkaloid, flavonoid, fenolik, dan saponin. Kadar flavonoid total yang diperoleh sebesar 105,322 ±0,04 mg QE/g ekstrak. Uji aktivitas antioksidan menggunakan metode ABTS menunjukkan bahwa ekstrak etanol daun A. evecta memiliki nilai IC50 sebesar 183,1 ppm, sedangkan kuersetin sebagai standar menunjukkan nilai IC50 sebesar 3,01 ppm. Hasil ini menunjukkan bahwa ekstrak etanol A. evecta memiliki aktivitas antioksidan yang lebih rendah dibandingkan kuersetin.

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Published

2025-12-31

How to Cite

Muzayinah, M., Wutsqa, Y. U., & Fauzi, F. M. (2025). Kadar Flavonoid Total dan Aktivitas Antioksidan Ekstrak Etanol Angiopteris evecta (G. Forst.) Hoffm. dengan Metode ABTS: Total Flavonoid Content and Antioxidant Activity of Ethanolic Extract of Angiopteris evecta (G. Forst.) Hoffm. Using the ABTS Method. Journal of Pharmacy and Nutrition Research, 1(02), 100–108. https://doi.org/10.64689/jopanur.v1i02.10

Issue

Vol. 1 No. 02 (2025): December, 2025

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Articles

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